Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals I. A Comparison of Isolation Procedures
نویسندگان
چکیده
Vacuoles were isolated from the upper epiderm is o f petals o f Petunia hybrida line R27 using three different procedures. Utilizing the vacuolar localization o f anthocyanin, vacuolar prep arations obtained through polybase treatment, osm otic shock and m echanical forces were com pared on purity, yield and stability. The comparison indicated that application o f the polybase procedure results in the best vacuolar preparations. Vacuoles could be obtained which m ain tained their acidic pH and retained their anthocyanin content.
منابع مشابه
Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals ü. Vacuolar Localization of Some Hydrolases
In vacuoles isolated from petal upper epidermis o f Petunia hybrida through polybase-induced lysis of protoplasts the vacuolar localization o f x-mannosidase and x-galactosidase was dem on strated. No association was observed between the tonoplast and these hydrolases. Protoplasts from different mutant lines blocked in anthocyanin biosynthesis were treated with DEAE-dextran to release vacuoles...
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